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EET 400 [ECU]    Theobroma cacao

Derivation: Estacion Experimental Tropical.
Location: E.E.T. Pichilingue, Ecuador.

Notes:  Some EET clones are imported genotypes which have been given the duplicate identifier [IMP], [POU] for kown Pound collections and [MIG] for known SAN MIGUEL clones.
 

Synonyms: ETS 400 [ECU]

Identified as an off-type (using SSR markers) in the IDIAF collection, Dominican Republic [EET-400]   IDIAF, 2011

    Disclaimer

Recommended seed parent in:
Colombia
Bi-parental cross: EET 400 [ECU] x IMC 67 
Recommended by: Instituto Colombiano Agropecuaria (ICA), Colombia
Year: 1980s (Until late 1990s)
 Reference
Recommended seed parent in:
Colombia
Bi-parental cross: EET 400 [ECU] x ICS 1 
Recommended by: Instituto Colombiano Agropecuaria (ICA), Colombia
Year: 1980s (Until late 1990s)
 Reference
Recommended seed parent in:
Colombia
Bi-parental cross: EET 400 [ECU] x ICS 39 
Recommended by: Instituto Colombiano Agropecuaria (ICA), Colombia
Year: 1980s (Until late 1990s)
 Reference
Recommended seed parent in:
Colombia
Bi-parental cross: EET 400 [ECU] x ICS 6 
Recommended by: Instituto Colombiano Agropecuaria (ICA), Colombia
Year: 1980s (Until late 1990s)
 Reference
Recommended seed parent in:
Colombia
Bi-parental cross: EET 400 [ECU] x ICS 60 
Recommended by: Instituto Colombiano Agropecuaria (ICA), Colombia
Year: 1980s (Until late 1990s)
 Reference

 Parents:
SILECIA 1 [ECU]  x Unknown
 - Selec. Patologia 7818
   Costa Rica - CATIE, 1981
 Mother to:
EQX 106 [EQX]    Trinidad - ICTA (EQX), No Date
EQX 51 [EQX]    Trinidad - ICTA (EQX), No Date
EQX 91 [EQX]    Trinidad - ICTA (EQX), No Date
EQX 92 [EQX]    Trinidad - ICTA (EQX), No Date
EQX 93 [EQX]    Trinidad - ICTA (EQX), No Date
GNV 105    Purdy, L.H. et al., 1998
GNV 22    Purdy, L.H. et al., 1998
TARS 27    Ricardo Goenaga et al., 2009
TARS 9    Ricardo Goenaga et al., 2009
 Father to:
EQX 20 [EQX]    Trinidad - ICTA (EQX), No Date
EQX 79 [EQX]    Trinidad - ICTA (EQX), No Date
EQX 83 [EQX]    Trinidad - ICTA (EQX), No Date
EQX 86 [EQX]    Trinidad - ICTA (EQX), No Date
EQX 96 [EQX]    Trinidad - ICTA (EQX), No Date

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 Reference
BioProject: PRJNA486011   NCBI   ENA FASTQ 
Project: PRJEB28591   EVA (project)   EVA (analysis) 
Library Source: Genomic
 
Run: SRR7774208   NCBI   ENA FASTQ 
Experiment: SRX4629616
BioSample: SAMN09934443
SampleName: EET_400
SampleSource: Costa Rica, Tree_ID: Tc17439
Run: SRR7774342   NCBI   ENA FASTQ 
Experiment: SRX4629482
BioSample: SAMN09934435
SampleName: EET400_Arbol-122
SampleSource: Ecuador, Tree_ID: Tc21291
 Reference
BioProject: PRJNA734904   NCBI   ENA FASTQ 
Library Source: Genomic
 
Run: SRR14743680   NCBI   ENA FASTQ 
Experiment: SRX11078442
BioSample: SAMN19549133
SampleName: EET400
SampleSource: AGROSAVIA, Colombia

 HELD IN

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List received in 2012   Reference
• Accession: 1004
   - Local Name: EET 400
• Accession: 1004
   - Local Name: EET 400
• Accession: 1004
   - Local Name: EET 400
List received in 1987   Reference
• Local Name: EET 400 [ECU]
List received in 2012   Reference
• Local Name: EET-400
   - Year of Accession: 1996
List received in 1990   Reference
• Local Name: EET 400 [ECU]
• Local Name: EET 400 [ECU]
List received in 2018   Reference
• Accession: 10290
   - Local Name: EET-400
   - Year of Accession: 1965
List received in 1987   Reference
• Local Name: EET 400 [ECU]
   - Year of Accession: 1974
List received in 2011   Reference
• Local Name: EET-400 (Incorrecto)
List received in 2012   Reference
• Local Name: EET- 400
List received in 2012   Reference
• Local Name: EET 400
   - Year of Accession: 1990
List received in 2012   Reference
• Local Name: EET400
 Honduras, Fundación Hondureña de Investigación Agrícola (FHIA)
List received in 1994   Reference
• Local Name: EET 400 [ECU]
List received in 2008   Reference
• Accession: IDN-13-TCAC-00537
   - Local Name: EET400
List received in 1990   Reference
• Local Name: EET 400 [ECU]
List received in 1995   Reference
• Local Name: EET 400 [ECU]
   - Year of Accession: 1987
List received in 2009   Reference
• Local Name: EET - 400
List received in 2011   Reference
• Local Name: EET 400
   - Year of Accession: 1965
List received in 2024   Reference
• Accession: PI 315302     GRIN-Global 
   - Local Name: EET 400 [ECU]
   - Year of Accession: 1939
List received in 2024   Reference
• Accession: MIA 30313     GRIN-Global 
   - Local Name: EET 400
   - Year of Accession: 1983
List received in 1972   Reference
• Local Name: EET 400 [ECU]

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Resistant
 Morera, J & Mora, A., 1990
Resistant
 Morera, J. et al., 1991
Tolerant
 Enriquez, G. & Soria, V.J., 1977
Susceptible, 100.00 % Infection
Note: External Severety = 4.4 and Internal Severety = 5.0
 Phillips, W., 1997
Moderately Susceptible, sp. P. palmivora (Pod).
Note: Unripe, Attached and not wounded
 Morera, J., 1993
Resistant, 12.00 % Infection, sp. P. palmivora (Pod).
Note: Unripe and not wounded
 Lopez-Baez, O. et al., 1996
Susceptible, sp. P. palmivora (Pod).
 Phillips, W., 1997
3.30 % Infection (Field Reaction).
Note: Severery of infection was High.. Sime Darby Plantations, Merlimau Estate.
 Lam, K.S., 1992
36.00 % Infection
Note: 15 plants sampled..
 Subali, S., 1992
Intermediate
 Fonseca, S.E.A & Albuquerque, P.S.B. de, 1996
Resistant
 Enriquez, G.A. & Soria, V.J., 1967
Tolerant
 Enriquez, G. & Soria, V.J., 1977

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Seed Index: 129.8 (dried beans per 100g)
Pod Index: 34.4 (pods per kg dried beans)
 Reference
Pod Index: 19.8 (pods per kg dried beans)
 Reference
Seed Index: 87.7 (dried beans per 100g)
Productivity: medium
 Reference
Seed Index: 118.3 (dried beans per 100g)
Pod Index: 31.1 (pods per kg dried beans)
 Reference
Acc: PI 315302
Pod Index: 39.1 (pods per kg dried beans)
Yield (dry beans per tree): 0.54 kg
 Reference
Pod Index: 50.3 (pods per kg dried beans)
Yield: 855 kg ha-1 yr-1
Yield (dry beans per tree): 0.57 kg
 Reference

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Number: 37.7
Total Dry Weight: 29.1 g
Dry Weight: 0.77 g
Note: 69 plants sampled
 Reference
Colour: dark purple
Shape: oblong
Number: 42.0
Total Wet Weight: 64.9 g
Cotyledon Dry Weight: 1.20 g
Cotyledon Length: 24.7 mm
Cotyledon Width: 12.3 mm
 Reference
Colour Dark Purple: Present
Colour Light Purple: Present
Colour Grey: Absent
Colour White: Present
Colour Mottled: Absent
Number: 32.2
Maximum Number: 48
Cotyledon Wet Weight: 1.65 g
Cotyledon Dry Weight: 1.14 g
Cotyledon Length: 25.5 mm
Cotyledon Width: 12.7 mm
Cotyledon Thickness: 7.9 mm
 Reference
Colour Purple: 37 %
Colour Light Purple: 34 %
Colour White: 19 %
Colour Mottled: 10 %
Shape: flat/elongate
Length: 26.5 mm
Width: 12.6 mm
Thickness: 9.3 mm
Cotyledon Wet Weight: 1.96 g
Cotyledon Dry Weight: 1.34 g
 Reference
Number: 37.8
Dry Weight: 0.84 g
 Reference
Acc: PI 315302
Number: 27.6
Total Wet Weight: 265.5 g
Total Dry Weight: 25.6 g
 Reference

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Butterfat Content: 54.40 %
Maximum Butterfat Content: 57.90 %
Minimum Butterfat Content: 52.05 %
Note: 18 plants sampled
 Reference
Testa: 4.80 %
 Reference
Butterfat Content: 52.40 %
Testa: 5.00 %
 Reference

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Size: large
 Reference
Shape: elliptical
Basal Constriction: moderate
Apex Form: obtuse
Rugosity: moderate
Length: 168.0 mm
Breadth: 79.0 mm
Colour: anthocyanin absent
Hardness: hard
Note: Hardness > 2.0 Mpa
 Reference
Basal Constriction: slight
Apex Form: slightly acute
Rugosity: moderate
Husk Thickness: 7.6 mm (9.7 - 5.5)
Length: 161.0 mm
Breadth: 76.0 mm
Colour: absent anthocyanin
Weight: 409.0 g
Pods Per Tree: 5
Hardness: moderate
Length Breadth Ratio: 2.11
Note: ratio of distances between ridges within a pair and between ridges of two different pairs = 0.78
 Reference
Shape: angoleta
Husk Thickness: 13.3 mm
Length: 156.0 mm
Breadth: 92.0 mm
Colour: yellow
Hardness: soft
Length Breadth Ratio: 2.05
 Reference
Acc: PI 315302
Length: 167.0 mm
Breadth: 74.0 mm
Weight: 335.3 g
Pods Per Tree: 21
 Reference
Shape: angoleta
Colour: green
 Reference

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Colour: slight anthocyanin
 Reference
Colour: light brown
 Reference
Colour: slight anthocyanin
 Reference

Length to Width Ratio: 2.67
Basal Angle: 161.0 °
Apical Angle: 92.0 °
 Reference

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Self Compatibility: self incompatible
 Reference
Ligule Colour: moderate anthocyanin
Stamen Colour: slight anthocyanin
Sepal Length: 7.55 mm
Ligule Width: 2.41 mm
Ovule Number: 45
Style Length: 2.57 mm
Pedicel Colour: green
 Reference
Ligule Colour: anthocyanin absent (apical)
Stamen Colour: anthocyanin absent
Sepal Colour: greyish green
Sepal Width: 2.29 mm
Sepal Length: 8.94 mm
Ligule Length: 7.49 mm
Ligule Width: 2.19 mm
Ovary Colour: anthocyanin absent (apical)
Ovary Width: 1.01 mm
Ovary Length: 1.22 mm
Style Length: 2.29 mm
Staminode Length: 7.85 mm
Pedicel Colour: yellowish white
Self Compatibility: self incompatible
Note: Translation of Methuen colour codes.
 Reference
Bud Colour: anthocyanin absent
Ligule Colour: anthocyanin absent
Stamen Colour: slight anthocyanin
Guide Line Length: 3.50 mm
Sepal Colour: anthocyanin absent
Sepal Width: 2.50 mm
Sepal Length: 7.90 mm
Sepal Length to Width Ratio: 3.22
Ligule Length: 3.60 mm
Ligule Width: 2.20 mm
Ligule Length to Width Ratio: 1.68
Ribbon Length: 2.70 mm
Ribbon Colour: anthocyanin absent
Ovary Colour: anthocyanin absent (apical), anthocyanin absent (basal)
Ovary Width: 1.20 mm
Ovary Length: 1.70 mm
Ovary Length to Width Ratio: 1.26
Ovule Number: 52
Style Colour: anthocyanin absent (apical), anthocyanin absent (basal)
Style Length: 2.50 mm
Staminode Colour: intense anthocyanin
Staminode Length: 7.00 mm
Pedicel Length: 17.10 mm
Pedicel Colour: slight anthocyani, slight anthocyani (ab), anthocyanin absent (basal)
Hairs on Upper Surface of Sepal: present
Pigmented Glands on Lower Surface of Sepal: present
Horns at Base of Guide Lines: absent
Pigmented Spot on Pollen Sac: absent
 Reference
Self Compatibility: self incompatible
 Reference
Self Compatibility: self incompatible
 Reference

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Phillips, W. (1997) Results of the artificial inoculation of Moniliophthora rorei using the Aspersion method. Unpublished data supplied by W. Phillips, CATIE, Costa Rica. Personal communication
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Subali, S. (1992) MARDI's results on performance of imported primary cocoa clones. In: Unpublished Proceedings of MCGC Mini-Seminar on the Performance of Imported Cocoa Clones, 13th October, 1992. Kuala Lumpur, Malaysia.
Fonseca, S.E.A & Albuquerque, P.S.B. de (1996) Avaliacao de clones de cacau na Amazonia Brasileira em relaçao a incidencia de vassoura-de bruxa. In: Proceedings of the 12th International Cocoa Research Conference, Salvador, Brazil, 1996. Cocoa Producers' Alliance.
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